Exception in thread "main" net.sf.samtools.SAMFormatException: SAM validation error: ERROR: Record 630636, Read name HWI-H212:69:C0NR3ACXX:1:1212:10100:13262, bin field of BAM record does not equal value computed based on alignment start and end, and length of sequence to which read is aligned
    at net.sf.samtools.SAMUtils.processValidationErrors(SAMUtils.java:448)
    at net.sf.samtools.BAMFileReader$BAMFileIterator.advance(BAMFileReader.java:541)
    at net.sf.samtools.BAMFileReader$BAMFileIterator.next(BAMFileReader.java:522)
    at net.sf.samtools.BAMFileReader$BAMFileIterator.next(BAMFileReader.java:481)
    at net.sf.samtools.SAMFileReader$AssertableIterator.next(SAMFileReader.java:672)
    at net.sf.samtools.SAMFileReader$AssertableIterator.next(SAMFileReader.java:650)
    at net.sf.picard.sam.MarkDuplicates.buildSortedReadEndLists(MarkDuplicates.java:397)
    at net.sf.picard.sam.MarkDuplicates.doWork(MarkDuplicates.java:161)
    at net.sf.picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:177)
    at net.sf.picard.sam.MarkDuplicates.main(MarkDuplicates.java:145) 
 
 
 
 

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